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Accueil > Research > Research teams > Regulation of terminal glycosylation > Publications

Regulation of terminal glycosylation




COLOMB F., KRZEWINSKI-RECCHI M.-A., STEENACKERS A., VINCENT A., HARDUIN-LEPERS A., DELANNOY P., GROUX-DEGROOTE S. TNF up-regulates ST3GAL4 and sialyl-LewisX expression in lung epithelial cells through an intronic ATF2 responsive element. Biochem. J. (2017) 474, 65-78. doi : 10.1042/BCJ20160602.


DEWALD J.H., COLOMB F., BOBOWSKI M., GROUX-DEGROOTE S., DELANNOY P. Role of Cytokine-Induced Glycosylation Changes in Regulating Cell Interactions and Cell Signaling in Inflammatory Diseases and Cancer. Cells (2016) 5, 43. doi:10.3390/cells5040043

SZUNERITS S., ZAGORODKO O., COGEZ V., DUMYCH T., CHALOPIN T., ALVAREZ DORTA D., SIVIGNON A., BARNICH N., HARDUIN-LEPERS A., LARROULET I., YANGUAS SERRANO A., SIRIWARDENA A., PESQUERA A., ZURUTUZA A., GOUIN S.G., BOUKHERROUB R., BOUCKAERT J. Differentiation of Crohn’s Disease-Associated Isolates from Other Pathogenic Escherichia coli by Fimbrial Adhesion under Shear Force. Biology (Basel) (2016) Apr 1 ;5(2). pii : E14. doi : 10.3390/biology5020014. https://www-ncbi-nlm-nih-gov.gate1....

DROLEZ A, VANDENHAUTE E, JULIEN S, GOSSELET F, BURCHELL J, CECCHELLI R, DELANNOY P, DEHOUCK MP, MYSIOREK C. Selection of a Relevant In Vitro Blood-Brain Barrier Model to Investigate Pro-Metastatic Features of Human Breast Cancer Cell Lines. PLoS One. 2016 Mar 9 ;11(3):e0151155. doi : 10.1371/journal.pone.0151155. eCollection 2016. https://www-ncbi-nlm-nih-gov.gate1i...

BAREL M., HARDUIN-LEPERS A., PORTIER L., SLOMIANNY M.-C., CHARBIT A. Host glycosylation pathways and the unfolded protein response contribute to the infection by Francisella. Cell Microbiol. (2016) May 17. doi : 10.1111/cmi.12614 (IF 4.915).

DROLEZ A, VANDENHAUTE E, DELANNOY CP, DEWALD JH, GOSSELET F, CECCHELLI R, JULIEN S, DEHOUCK MP, DELANNOY P, MYSIOREK C. ST6GALNAC5 Expression Decreases the Interactions between Breast Cancer Cells and the Human Blood-Brain Barrier. Int J Mol Sci. 2016 Aug 11 ;17(8). pii : E1309. doi : 10.3390/ijms17081309.

TEPPA E., PETIT D., PLECHAKOVA O., COGEZ V., HARDUIN-LEPERS A. Phylogenetic-derived insights into the evolution of sialylation in Eukaryotes : Comprehensive analysis of Vertebrate β-galactoside α2,3/6-sialyltransferases (ST3Gal and ST6Gal) Int. J. Mol. Sci. (2016) 17, 1286 invited review in special issue “glycan receptor interaction (Ed Cheorl Ho Kim) doi:10.3390/ijms17081286. https://www-ncbi-nlm-nih-gov.gate1....

WOODMAN N, PINDER SE, TAJADURA V, LE BOURHIS X, GILLETT C, DELANNOY P, BURCHELL JM, JULIEN S. Two E-selectin ligands, BST-2 and LGALS3BP, predict metastasis and poor survival of ER-negative breast cancer. Int J Oncol. 2016 Jul ;49(1):265-75. doi : 10.3892/ijo.2016.3521.

DELANNOY C. ; ROMBOUTS Y., GROUX-DEGROOTE S., HOLST S., CODDEVILLE B., HARDUIN-LEPERS A. ; WUHRER M., ELASS-ROCHARD E., GUERARDEL Y. Glycosylation changes triggered by the differentiation of monocytic THP-1 cell line into macrophages J. Prot. Res. (2016) Jun 28.

GILORMINI P.-A., LION C., NOEL M., KRZEWINSKI-RECCHI M.-A., HARDUIN-LEPERS A., GUERARDEL Y, BIOT C. Improved workflow for the efficient preparation of ready to use CMP-activated sialic acid (2016) Glycobiology (IF 3.283). DOI:10.1093/glycob/cww084.

VIDEIRA PA, JULIEN S, LOUREIRO LR, SOUSA DP, CARRASCAL MA, DELANNOY P. Vaccine and antibody therapy against Thomsen–Friedenreich Tumor-Associated Carbohydrate Antigens. In “ Recent Advances in Biotechnology : Recent Progress in Glycotherapy ” Zhou Q. ed., Bentham Science Publishers, Oak Park, IL, USA, 2016, pp. 3-38.


VANDERMEERSCH S., VANBESELAERE J., DELANNOY C.P., DROLEZ A., MYZIOREK C., GUÉRARDEL Y., JULIEN S., DELANNOY P. Accumulation of GD1 ganglioside in MDA-MB-231 breast cancer cells expressing ST6GalNAc V. Molecules (2015) 20, 6913-6924. doi:10.3390/molecules20046913.

GUÉRARDEL Y., GROUX-DEGROOTE S., DELANNOY P. Gangliosphyngolipids : their structure and biological roles. In “Carbohydrate Chemistry : State-of-the-art and challenges for drug development” Cipolla L. ed., Imperial College Press, London, UK (2015) pp. 35-56.

PETIT D., TEPPA R.E., MIR A.-M., VICOGNE D., THISSE C., THISSE B., FILLOUX C. and HARDUIN-LEPERS A. Integrative view of ß–galactoside a2,3-sialyltransferases (ST3Gal) evolutionary relationships and functional divergence in Chordates : Massive st3gal gene loss in Homo sapiens. Mol Biol Evol. 2015, 32(4):906-27. doi : 10.1093/molbev/msu395.

GROUX-DEGROOTE S., GUÉRARDEL Y., JULIEN S., DELANNOY P. Gangliosides in Breast Cancer : New Perspectives. Biochemistry (Mosc). 2015 ; 80(7) : 808-19

MIHALACHE A., DELPLANQUE J.F., RINGOT-DESTREZ B., WAVELET C., GOSSET P., NUNES B., GROUX-DEGROOTE S., LÉONARD R., ROBBE-MASSELOT C. Structural Characterization of Mucin O-Glycosylation May Provide Important Information to Help Prevent Colorectal Tumor Recurrence. Front Oncol. 2015, 5 : 217

GERARDY-SCHAHN R, DELANNOY P, VON ITZSTEIN M. Preface. Top Curr Chem. 2015, 367:v-vii

LOUREIRO L.R., CARRASCAL M.A., BARBAS A., RAMALHO J.S., NOVO C., DELANNOY P., VIDEIRA P.A. Challenges in Antibody Development against Tn and Sialyl-Tn Antigens. Biomolecules. 2015 11 ;5(3) : 1783-809. doi : 10.3390/biom5031783

CIME-CASTILLO J.A., DELANNOY P., MENDOZA-HERNANDEZ G., MONRROY-MARTINEZ V., HARDUIN-LEPERS A., LANZ-MENDOZA H., HERNANDEZ-HERNANDEZ F., ZENTENO-GALINDO E. CABELLO-GUTIERREZ C., RUIZ-ORDAZ B.H. Sialic Acid Expression in the Mosquito Aedes aegypti and its Possible Role in Dengue Virus-Vector Interactions. Biomed Res Int. (2015) 2015 : 504187.


COLOMB F., VIDAL O., BOBOWSKI M., KRZEWINSKI-RECCHI M.-A., HARDUIN-LEPERS A., MENSIER E., JAILLARD S., LAFITTE J.-J., DELANNOY P. and GROUX-DEGROOTE S. TNF induces the expression of ST3Gal IV in human bronchial mucosa via MSK1/2 protein kinases and increases FliD/sialyl-Lewisx mediated adhesion of Pseudomonas aeruginosa. Biochem. J. 2014, 457(1), 79-87. doi : 10.1042/BJ20130989.

DALL’OLIO F., MALAGOLINI N., CHIRICOLO M., TRINCHERA M. and HARDUIN-LEPERS A. The expanding roles of the Sda/Cad carbohydrate antigen and its cognate glycosyltransferase B4GALNT2. Biochim. Biophys. Acta- General Subjects 2014, 1840(1), 443-453. doi : 10.1016/j.bbagen.2013.09.036.

GROUX-DEGROOTE S, WAVELET C, KRZEWINSKI-RECCHI MA, PORTIER L, MORTUAIRE M, MIHALACHE A, TRINCHERA M, DELANNOY P, MALAGOLINI N, CHIRICOLO M, DALL’OLIO F, HARDUIN-LEPERS A. B4GALNT2 gene expression controls the biosynthesis of Sd(a) and sialyl Lewis X antigens in healthy and cancer human gastrointestinal tract. Int J Biochem Cell Biol. 2014, 442-9. doi : 10.1016/j.biocel.2014.06.009.

VAN SLAMBROUCK S, GROUX-DEGROOTE S, KRZEWINSKI-RECCHI MA, CAZET A, DELANNOY P, STEELANT WF. Carbohydrate-to-carbohydrate interactions between a2,3-linked sialic acids on a2 integrin subunits and asialo-GM1 underlie the bone metastatic behaviour of LNCAP-derivative C4-2B prostate cancer cells. Biosci Rep. 2014, 34(5). pii : e00138. doi : 10.1042/BSR20140096.

JULIANT S., HARDUIN-LEPERS A., MONJARET F., CATIEAU B., -VIOLET M.-L., CÉRUTTI P., OZIL A., DUONOR-CÉRUTTI M. Isolation and characterization of the ?1,6-fucosyltransferase gene from the lepidopteran insect cell line Sf9 : insights into fut8 gene evolution in animals. PlosOne 2014, 9, (10) e110422 doi:10.1371/journal.pone.0110422.t001

JULIEN S., DELANNOY P. Sialic Acid and Cancer. In : Taniguchi N., Endo T., Hart G., Seeberger P., Wong C. (Eds.) Glycoscience : Biology and Medicine (2014) SpringerReference ( Springer Japan, pp. 1419-1424.

COLOMB F., ROBBE-MASSELOT C., GROUX-DEGROOTE S., BOUCKAERT J., DELANNOY P., MICHALSKI J.C. Epithelial Mucins and Bacterial Adhesion. Carbohydr. Chem. (2014) 40, 596–623.


HARDUIN-LEPERS A. Vertebrate sialyltransferases. In : Sialobiology : Structure, Biosynthesis and Function. Sialic acid glycoconjugates in health and diseases. Ed. : J. Tiralongo and I Martinez-Duncker, 2013, Chapter 5, pp. 139-187. doi : 10.2174/97816080538651130101

BOBOWSKI M, VINCENT A, STEENACKERS A, COLOMB F, VAN SEUNINGEN I, JULIEN S, DELANNOY P. Estradiol Represses the GD3 Synthase Gene ST8SIA1 Expression in Human Breast Cancer Cells by Preventing NF ?B Binding to ST8SIA1 Promoter. PLoS One. 2013, 23 ;8(4):e62559.

HOTTIN A, WRIGHT DW, STEENACKERS A, DELANNOY P, DUBAR F, BIOT C, DAVIES GJ, BEHR JB. a-L-Fucosidase Inhibition by Pyrrolidine-Ferrocene hybrids : Rationalization of Ligand Binding Properties by Structural Studies. Chem. 2013, 19(29), 9526-33. doi : 10.1002/chem.201301001. IF : 5.92

PETIT D., TEPPA R.E., PETIT J.-M., HARDUIN-LEPERS A. A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of Golgi-glycosyltransferases. Methods in molecular biology, Glycobiology Protocols (2013) 1022, 73-97. doi : 10.1007/978-1-62703-465-4_7.

JULIEN S, BOBOWSKI M, STEENACKERS A, LE BOURHIS X, DELANNOY P. How Do Gangliosides Regulate RTKs Signaling ? Cells. 2013, 2(4):751-67. doi : 10.3390/cells2040751.

MARTINEZ P, VERGOTEN G, COLOMB F, BOBOWSKI M, STEENACKERS A, CARPENTIER M, ALLAIN F, DELANNOY P, JULIEN S. Over-sulfated glycosaminoglycans are alternative selectin ligands : insights into molecular interactions and possible role in breast cancer metastasis. Clin Exp Metastas 2013, 30(7):919-31. doi : 10.1007/s10585-013-9592-7.

JULIANT S., LEVEQUE M., CERUTTI P., OZIL A., CHOBLET S., VIOLET M.-L, SLOMIANNY M.-C., HARDUIN-LEPERS A. and CERUTTI M. Engineering baculovirus genome to produce galactosylated antibodies in lepidopteran cells. Methods in Molecular Biology, Glycobiology Protocols 2013, 988, 59-77. doi : 10.1007/978-1-62703-327-5_5.

BOBOWSKI M., HARDUIN-LEPERS, DELANNOY P. ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1). in Handbook of Glycosyltransferases and Related Genes, Naoyuki Taniguchi, Koichi Honke, Minoru Fukuda, Hisashi Narimatsu, Yoshiki Yamaguchi and Takashi Angata, eds. © Sprinter-Verlag Berlin Heidelberg (2013) doi : 10.1007/SpringerReference_332175


HARDUIN-LEPERS A., KRZEWINSKI-RECCHI M.A., COLOMB F., FOULQUIER F., GROUX-DEGROOTE S. and DELANNOY, P. Sialyltransferases function in cancer. Front. Biosci. 2012, 4, 499-515.

GUÉRARDEL Y., CHANG L.-Y., FUJITA A., CODDEVILLE B., MAES E., SATO C., HARDUIN-LEPERS A., KUBOKAWA K. and KITAJIMA K. Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution. Glycobiology 2012, 4 (22), 479-491.

VANBESELAERE, J., CHANG L.-Y., HARDUIN-LEPERS A., FABRE E., SLOMMIANNY, C., BIOT C. KHOO K.-H. and GUÉRARDEL Y. Zebrafish liver cells is a coherent model for the study of glycosylation in zebrafish. Journal of proteome research 2012, 11, 2164-2177.

JULIEN S, VIDEIRA PA, DELANNOY P. Sialyl-Tn in cancer , (how) did we miss the target ? Biomolecules 2012, 2, 435-466. doi, 10.3390/biom2040435.

CAZET A, BOBOWSKI M, ROMBOUTS Y, LEFEBVRE J, STEENACKERS A, POPA I, GUÉRARDEL Y, LE BOURHIS X, TULASNE D, DELANNOY P. The ganglioside G(D2) induces the constitutive activation of c-Met in MDA-MB-231 breast cancer cells expressing the G(D3) synthase. Glycobiology. 2012, 22(6), 806-16.

STEENACKERS A, CAZET A, BOBOWSKI M, ROMBOUTS Y, LEFEBVRE J, GUÉRARDEL Y, TULASNE D, LE BOURHIS X, DELANNOY P. Expression of GD3 synthase modifies ganglioside profile and increases migration of MCF-7 breast cancer cells. C. R. Chimie 2012, 15, 3-14.

HOTTIN A, DUBAR F, STEENACKERS A, DELANNOY P, BIOT C, BEHR JB. Iminosugar-ferrocene conjugates as potential anticancer agents. Org Biomol Chem. 2012, 10(29), 5592-7. doi, 10.1039/c2ob25727k.

STEENACKERS A, VANBESELAERE J, CAZET A, BOBOWSKI M, ROMBOUTS Y, COLOMB F, LE BOURHIS X, GUÉRARDEL Y, DELANNOY P. Accumulation of unusual gangliosides G(Q3) and G(P3) in breast cancer cells expressing the G(D3) synthase. Molecules. 2012, 17(8), 9559-72.

BOBOWSKI M, CAZET A, STEENACKERS A, DELANNOY P. Role of complex gangliosides in cancer progression. Carbohydr. Chem. 2012, 37, 1-20.

COLOMB F., KRZEWINSKI-RECCHI M.-A., El MACHHOUR F., MENSIER E., JAILLARD S., STEENACKERS A., HARDUIN-LEPERS A., LAFITTE J.-J. DELANNOY P. and GROUX-DEGROOTE S. TNF- ? enhances the expression of sialyl-Lewisx and 6-sulfo-sialyl-Lewisx carbohydrate antigens in human A549 cells and bronchial mucosa through the up-regulation of ST3GAL4 specific transcript. Biochimie 2012, 94, 2045-2053.


AUDRY M., JEANNEAU C., IMBERTY A., HARDUIN-LEPERS A., DELANNOY, P. and BRETON C. Current trends in the structure-activity relationships of the sialyltransferases. Glycobiology 2011, 21 (6), 716-726 . doi, 10.1093/glycob/cwq189.

TEYLAERT B., MEURICE E., BOBOWSKI M., HARDUIN-LEPERS A., GAUCHER C., FONTAYNE A., JORIEUX S. and DELANNOY P. Molecular cloning, characterization, genomic organization and promoter analysis of the alpha1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0 BMC Biotechnology 2011, 11, 1. doi, 10.1186/1472-6750-11-1.

VAN DER POEL S, WOLTHOORN J, VAN DEN HEUVEL D, EGMOND M, GROUX-DEGROOTE S, NEUMANN S, GERRITSEN H, VAN MEER G, SPRONG H. Hyperacidification of trans-Golgi network and endo/lysosomes in melanocytes by glucosylceramide-dependent V-ATPase activity. Traffic. 2011, 12(11), 1634-47.

JULIEN S, IVETIC A, GRIGORIADIS A, QIZE D, BURFORD B, SPROVIERO D, PICCO G, GILLETT C, PAPP SL, SCHAFFER L, TUTT A, TAYLOR-PAPADIMITRIOU J, PINDER SE, BURCHELL JM. Selectin ligand sialyl-Lewis x antigen drives metastasis of hormone-dependent breast cancers. Cancer Res. 2011, 71(24), 7683-93.


LEHOUX S, GROUX-DEGROOTE S, CAZET A, DHAENENS CM, MAURAGE CA, CAILLET-BOUDIN ML, DELANNOY P, KRZEWINSKI-RECCHI MA. Transcriptional regulation of the human ST6GAL2 gene in cerebral cortex and neuronal cells. Glycoconj J. 2010, 27(1), 99-114.

BERNARDIN A, CAZET A, GUYON L, DELANNOY P, VINET F, BONNAFFE D, TEXIER I. Copper-free click chemistry for highly luminescent quantum dot conjugates, application to in vivo metabolic imaging. Bioconjug Chem. 2010, 21(4), 583-8.

CARVALHO A.S., HARDUIN-LEPERS A., MAGALHÃES A., MACHADO E., MENDES N., COSTA L.T., MATTHIESEN R., ALMEIDA R., COSTA J., REIS C.A. Differential expression of alpha-2,3-sialyltransferases and alpha-1,3/4-fucosyltransferases regulates the levels of sialyl Lewis a and sialyl Lewis x in gastrointestinal carcinoma cells. Int J Biochem Cell Biol. 2010, 42, 80-89.

HARDUIN-LEPERS A. Comprehensive analysis of sialyltransferases in vertebrate genomes. Glycobiology Insights 2010, 2, 29-61. doi, 10.4137/GBI.S3123.

CAZET A., JULIEN S., BOBOWSKI M., KRZEWINSKI-RECCHI M.-A., HARDUIN-LEPERS A., GROUX-DEGROOTE S., DELANNOY P. Consequences of the expression of sialylated antigens in breast cancer. Carbohydr Res. 2010, 45(10), 1377-1383. ,

CAZET A, JULIEN S, BOBOWSKI M, BURCHELL J, DELANNOY P. Tumour-associated carbohydrate antigens in breast cancer. Breast Cancer Res. 2010, 12(3), 204.

CAZET A, LEFEBVRE J, ADRIAENSSENS E, JULIEN S, BOBOWSKI M, GRIGORIADIS A, TUTT A, TULASNE D, LE BOURHIS X, DELANNOY P. GD3 synthase expression enhances proliferation and tumor growth of MDA-MB-231 breast cancer cells through c-Met activation. Mol Cancer Res. 2010, 8(11), 1526-35.

PETIT D., MIR A.-M., THISSE C., DELANNOY P., ORIOL R., THISSE B. and HARDUIN-LEPERS A. Molecular phylogeny and functional genomics of ß–galactoside a2,6-sialyltransferases to explain ubiquitous expression of st6gal1 in Amniotes. J. Biol. Chem. 2010, 285, 38399-38414. doi, 10.1074/jbc.M110.163931.


DELANNOY P, TEYLAERT B, JORIEUX S, MEURICE E, GAUCHER C, FONTAYNE A, BOBOWSKI M. Utilisation d’un répresseur d’un gène codant une enzyme possédant une activité glycosyltransferase. Brevet n° 10 50857, déposé le 8 février 2010.

PhD thesis

AURELIE CAZET : Effet de l’expression de la GD3 synthétase sur la progression et l’agressivité du cancer du sein.
8/12/2010 (UST Lille1)

MARIE BOBOWSKI : Régulation transcriptionnelle et rôle de la GD3 synthétase, enzyme clef de la biosynthèse des gangliosides, dans le cancer du sein.
14/12/2012 (UST Lille1)

FLORENT COLOMB : Régulation de la biosynthèse des épitopes sialyl-Lewisx et 6-sulfo-sialyl-Lewisx par el TNF dans la muqueuse bronchique humaine et influence sur l’adhésion de Pseudomonas aeruginosa.
17/12/2012 (UST Lille1)

AGATA STEENACKERS : Etude du rôle des gangliosides complexes dans la prolifération des cellules de cancer du sein.
28/11/2013 (UST Lille1)

ADRIANA MIHALACHE : Recherche de nouveaux marqueurs diagnostiques et/ou pronostiques pour le cancer du colon.
11/01/2016 (UST Lille1)

Cindy WAVELET : Régulation transcriptionnelle de la B4GALNT2 humaine dans le colon.
16/12/2016 (UST Lille1)

HDR thesis

MARIE-ANGE KRZEWINSKI-RECCHI : Les sialyltransférases au « sein » des cancers.
19/02/2010 (UST Lille1).

SOPHIE GROUX-DEGROOTE : Anomalies de la glycosylation terminale dans la mucoviscidose et les cancers : régulation des glycosyltransférases.
7/07/2011 (UST Lille1)