Role and biosynthesis of osmoregulated periplasmic glucans (OPG) in Proteobacteria
The team works on biosynthesis and function of bacterial periplasmic glucans. These molecules are one of the essentials virulent factors required for bacterial pathogens. Periplasmic glucans are required for proper interaction between bacteria and their hosts (human, animal or vegetable). They participate to the cell signalling as an intermediate in the perception of environmental changes. Their absence leads to an inappropriate response because the bacteria are unable to recognize their environment properly. Experiments are actually performed on phytopathogen (Dickeya dadantii) and on zoopathogen (Escherichia coli, Yersinia pseudotuberculosis and Yersinia pestis) bacterial species. Elucidation of the function of the glucans needs genetic, biochemical and molecular biology approaches. These glucans are potential targets for new antibacterial molecules. This kind of molecules will lead to inhibition of growth of pathogens within their host.
strains of the phytopathogen D. dadantii on chicory leaves
Dr. Florent Sebbane ( INSERM U1019, Institut Pasteur de Lille, CIIL, Lille)
Inhibition of bacterial/host interactions.
Among essential steps of bacterial virulence are interaction with host cells (colonization and internalization) and biofilm formation. Decreasing or blocking these steps may prevent infection.
We synthesize and screen molecular glycomimetics inhibiting interactions between pRRs of the eukaryotic host membrane surface and their corresponding envelope bacterial PAMPs, to assess their selectivity and their mechanism of action in vitro and in vivo. Our models are E. coli and Pseudomonas aeruginosa.
and its inhibition by one glycomimetic (+glyM, right)
Two ANRs « PA-Control » and “Glycomimes” in collaboration with a consortium of biologists, chemists and biotechnology industry.
UGSF partner :
Pr. Gérard Vergoten (Team : Structural diversity of heparan sulphate and regulation of the C response)
Externals partners :
Pr. Pierre Desreumaux (INSERM U995, CHRU, Lille)
Dr. Jean-Jacques Vasseur, Dr. François Morvan (IBMM, UMR CNRS 5247, Montpellier)
Dr. Benoît Darblade (Society Elicityl, Grenoble)
Dr. Yann Chevolot, Dr. Eliane Southeyrand (INL, CNRS UMR 5270, Lyon)
Dr. Sebastien Vidal (ICBMS-UCBL, UMR CNRS 5246, Lyon)
Key-words : pathogen bacterial envelope, bacteria/environment or host interactions, periplasmic glucans, two-component systems, biofilms, synthetic antibacterial molecules.